Evaluation of Novel Chip Based Test - Truenat with Traditional Genexpert for Detection of Tubercular Meningitis

Anurag Agarwal

Department of Pediatrics Maulana Azad Medical College and Lok Nayak Hospital, New Delhi -110002, India.

Stuti Sharma *

Department of Pediatrics Maulana Azad Medical College and Lok Nayak Hospital, New Delhi -110002, India.

Ashwani Khanna

Lok Nayak Hospital. New Delhi -110002, India.

Raghvendra Singh

Department of Pediatrics Maulana Azad Medical College and Lok Nayak Hospital, New Delhi -110002, India.

Reema Arora

Lok Nayak Hospital. New Delhi -110002, India.

*Author to whom correspondence should be addressed.


Abstract

Background: TRUENAT is a novel chip based test developed in India. Its use for pulmonary samples has been approved by World Health Organisation. Government of India has recently approved it for Extrapulmonary cases.

Aim: To study the role of TRUENAT in diagnosis of Tubercular Meningitis(TBM) and the co-relation between TRUENAT and GeneXpert.

Study Design: Prospective study.

Subjects and Methods: 75 Children and adolescents with strongly suggestive TBM as per NTEP guidelines [1] were enrolled. Clinical, Radiological and Cerebrospinal fluid(CSF) analysis were carried out. CSF was tested by TRUENAT and GeneXpert.

Results: TRUENAT detected 13/75 cases in comparison to GeneXpert which detected 16/75 cases. Out of these, in only 4 samples MTB was detected by both these tests. Concordance between the two tests was 72%. Cohen kappa (k-0.105) analysis showed slight agreement between the two tests. Higher CSF count(>10cell) was associated with higher chances of positivity in GeneXpert as well as TRUENAT.

Conclusion: TBM diagnosis requires a combination of clinical, radiological and biochemical parameters. Molecular tests alone cannot detect bacilli in many cases. A single molecular test which targets additional genes could provide higher detection.

Keywords: Cerebrospinal fluid, genexpert, pediatrics, truenat, tubercular meningitis


How to Cite

Agarwal, Anurag, Stuti Sharma, Ashwani Khanna, Raghvendra Singh, and Reema Arora. 2024. “Evaluation of Novel Chip Based Test - Truenat With Traditional Genexpert for Detection of Tubercular Meningitis”. Asian Journal of Pediatric Research 14 (7):47-53. https://doi.org/10.9734/ajpr/2024/v14i7364.

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